Bacteriology
Nutrient agar recipe
Bacterial cell fixation
Parasitology
Leishmania culture
Drug recipes for Drug resistant L. donovani strains
EC50
Exosome extraction
Molecular biology
Plasmid extraction (Gene Jet Kit)
Transformation – Nucleofection
RNA extraction from mammalian cells
DNAse treatment for RNA extracted from mammalian cells
RNA cleanup after DNAse treatment
cDNA synthesis from RNA
RT-qPCR
Transformation – conjugation
Plasmid extraction (phenol/chloroform)
Genomic DNA extraction (phenol/chloroform)
Nanodrop procedure
In vitro cell assays
Cell culture infection with Leishmania
LPS preparation
Biochemical assays
Griess reaction
MTT
Fluorescence assays